【文獻標題】Dehydroepiandrosterone reduces accumulation of lipid droplets in primary chicken hepatocytes by biotransformation mediated via the cAMP/PKA-ERK1/2 signaling pathway

【作者】Longlong Li, Chongyang Ge, Dian Wang, et.al

【作者單位】南京農(nóng)業(yè)大學（Nanjing Agricultural University）

【文獻中引用產(chǎn)品】

雞脫氫表雄酮（DHEA）ELISA試劑盒

【關鍵詞】Dehydroepiandrosterone，F(xiàn)at accumulation，Biotransformation，cAMP/PKA-ERK1/2 signaling pathway，Primary chicken hepatocytes

【DOI】doi.org/10.1016/j.bbalip.2018.03.007

【影響因子(IF)】5.03

【出版期刊】《BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS》

【產(chǎn)品原文引用】

Rabbit anti-17β-HSD and anti-aromatase antibodies were purchased from Abcam (Abcam, USA). Rabbit anti-AMPKα, phospho-AMPKα,ACCα, phospho-ACCα, ERK1/2, phospho-ERK1/2 and β-actin were purchased from Cell Signaling Technology (Boston, MA, USA). Goat anti-rabbit IgG horseradish peroxidase conjugate was purchased from Boster (Wuhan, China). U0126-EtOH (phospho-ERK1/2 inhibitor),Forskolin (PKA activator), H89 (PKA inhibitor), Trilostane (3β-HSD inhibitor), Flutamide (androgen receptor [AR] antagonist), and Fulvestrant (estrogen receptor [ER] antagonist) were purchased from Selleck Chemicals (Houston, TX, USA). 12-O-tetradecanoyl phorbol-13-acetate (TPA, an ERK activator) was purchased from ApexBio (Houston,USA). Protein assay kits were obtained from the Beyotime Biotechnology Institute (Shanghai, China). DHEA Enzyme-linked immunosorbent assay (ELISA) kits were purchased from Hengyuan Biological Technology Co. (Shanghai, China). Testosterone and estradiol radioimmunoassay (RIA) kits were purchased from the Beijing Beifang Biotechnology Institution (Beijing, China). DHEA, estradiol, or testosterone were dissolved in DMSO and stored at 20℃. The working solutions of DHEA, estradiol, or testosterone containing of DMSO were diluted in M199 medium, and the DMSO content in all working solutions did not exceed 0.1% by volume.

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